, a serum free formulation with no animal compound:

	• nutritive solution with carbon hydrates, metabolic precursors, minerals, vitamins, hepes buffer, phenol red
	• and antibiotics: streptomycin, penicillin, amphotericin B.

, a serum free formulation with no dextran:

• nutritive solution with poloxamer.

Outcome of keratoplasty for the recipient, is linked to endothelial cells (EC) density.

saves additional 42% of EC's compared to serum supplemented medium (serum medium)*.

Need for qualified grafts shall be fulfilled

saves additional 40% graft compared to serum supplemented medium (serum medium)*.

De-swelling is an important issue

guarantees the right thickness and preserves the endothelium*.

One NEST'IN vial is used to transport the cornea from the excision site to the eyebank.
After initial inspection, the graft is placed in a new NEST'IN vial for 21 days.
Two days before keratoplasty, the graft is placed in NEST'OUT for de-swelling and transportation.

Before use:

	• storage of the media at - 20°C for 18 months or at + 4°C for 12 months.
	• transport, at ambient temperature up to 25°C, alternatively at controlled temperature between + 2°C to + 8°C.

With the graft:

	• optimum storage temperature: + 31°C.
	• transport before and after organ culture at ambient temperature up to 25°C (1 day) alternatively at controlled temperature between + 2°C to + 8°C (2 to 3 days).

	• The graft can be stored in NEST'IN without any suture (no contamination, no epithelial cells proliferation).
	• The graft endothelium can be visualized after de-swelling without using osmotic dilation, NEST'OUT facilitates the EC's count operation.
	• A security seal guarantees the inviolability instead of a crimp cap.
	• Storage at + 4°C before use saves time for thawing and reaching use temperature: avoiding thermal stress between solution and graft is a key success factor.

Increased productivity of qualified grafts boosts savings for the eye bank.