Arcadophta Monoblue : Sterile solution of purified Trypan Blue

Intraocular tissues staining

Vital staining

Bibliography

Sterile solution of purified Trypan Blue

Presentation :
Monoblue 0,055% : 0,75 ml ready-to-use syringe
Monoblue 0,25% : 1ml ready-to-use syringe

increases the safety of Trypan Blue use thanks to its raw material purity.

Trypan Blue toxicity is described

	• Mutagenicity of benzidine and benzidine-congener dyes and selected monoazo dyes in a modified Salmonella assay.A.J.Prival 1984
	• Biocompatibility of trypan blue with human corneal cells. BTH.Van dooren 2004

Eye Toxicity

	• Neurotoxic effects of trypan blue on rat retinal ganglion cells. Department of Ophthalmology, University of Tokyo School of Medicine 2005
	• Retinal tolerance to dyes. Center of Ophthalmology, University of Cologne 2005
	• Toxic effects of indocyanine green, infracyanine green, and trypan blue on the human retinal pigmented epithelium. Department of Ophthalmology, Inselspital, University of Bern 2005
	• Trypan blue: effect on retinal pigment epithelial and neurosensory retinal cells. Department of Ophthalmology, University of California, Irvine 2005

, an unmatched Trypan Blue purity of more than 95% to reduce Trypan Blue toxicity.

Minimize the risks due to impurities (purple, mono-azo component)

Allows to reduce the active component concentration.

O-tolidine free (no carcinogenic consequences).

Standard Trypan Blue VS Monoblue

increases the safety of Trypan Blue use thanks to its presentation in prefilled syringe.

Monoblue 0,055% Various applications for an exclusive anterior segment use.

    Cataract / Visualization of crystalline epithelial cells
• Dye-enhanced phakoemulsification: learning hydrodissection and hydrodelineation technique. Werner L pandey sk .2000.
• Trypan blue assisted posterior plaque removal. Namrata Sharma, Wishal Gupta 2002.
• A novel use of trypan blue to minimize epithelial cell proliferation in pediatric cataract surgery. A.W.Kiel.2003.

    Glaucoma
• Trypan blue identifies antimetabolite treatment area. Healey Pr Crowston Jg. 2005.
• Use of trypanblue to confirm the patency of filtering surgery. Shishir Agrawal Jaya Agrawal. 2005.

    Cornea
• Use of trypan blue for penetrating keratoplasty. Jonhatan Cp Roos Malcom g.
• Deep lamellar keratoplasty with trypan blue intrastromal staining. E.B.Balestrazzi. 2002.
• Trypan Blue assisted descemetorhexis for inadvertently retained Descemet's membrane after penetrating keratoplasty. R.Sinha.2003

    Other staining applications
• Vital staining of corneal endothelium in cataract extraction. Norn .1971
• Staining caracteristics of preserved human amniotic membrane. D.J.Hu .2003
• Visualisation of conjonctival cyst using healon v and trypan blue. Kobayashi A , Sugiyama. 2005.
• Staining tenon capsule with trypan blue during enucleationsurgery. Leanne M Cheung ,Geoffrey A.
• Staining characteristics of preserved human amniotic membrane. Daniel J Hu ,Surendra Basti. 2003.
• Trypan blue to aid visualization of the vitreous during anterior segment surgery. Cacciatori M, Chadha V, Bennett HG. 2006.

Monoblue 0,25% A well known application

Determination of the corneal graft viability
• Analyse séquentielle de l'endothelium cornéen humain in vitro. B.Delbosc. 1988.
• Contamination of trypan blue with Burkholderia cepacia in a cornea bank. Unite de Therapie Cellulaire et Genique, Etablissement Francais du Sang, Besancon.

A presentation in line with the French health authorities (AFSSAPS) requirements

Bulletin vigilance Hostmat, Décembre 2001.